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Fig. 4
Larvae infected with Salmonella during light exposure demonstrate enhanced bacterial clearance. (a) Live imaging of larvae infected with GFP-labeled Salmonella, at 3 and 24 hpi under light/dark, constant light, dark/light and constant dark conditions. Inset, magnified view of boxed region. (b) CFU counts of GFP-labeled Salmonella within individual infected larvae raised under light/dark, constant light, dark/light and constant dark conditions. (c) CFU measurements of GFP-labeled Salmonella in Luria Bertani broth grown under light/dark, constant light, dark/light and constant dark conditions over a 24- hour period. Scale bar, 500 μm and 100 μm in inset; hpi, hours post-infection; data in (c) represent three biological replicates; data shown as mean ± s.e.m. in (b) and s.d. in (c); p-values were calculated by one-way ANOVA with Tukey’s multiple comparisons test; ns, not significant.