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Fig. 3 Incus and retrotympanic defects in Jag1; Twist1 compound mutants. (a) Dissected incus bones of three-week-old mice stained with Alizarin Red S. Wild types and this Jag1 +/− example display a normal incus. In contrast, Twist1 +/− and Jag1 +/−; Twist1 +/− mice have an extra process (black arrows) extending from the anterior medial edge of the incus body. Accompanying diagrams illustrate the ectopic processes with orange lines. (b) Views of the temporal bone in three-week-old mice stained with Alizarin Red S. The dashed box in the illustration shows the approximate region being imaged. Compared to wild-type and Jag1 +/− mice, Twist1 +/− and Jag1 +/−; Twist1 +/− mice display reduction and fragmentation of the retrotympanic process (shown in orange in the adjacent diagrams).