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Fig. 4
Aggregation susceptibility of mutant GFAPs can be assessed using zebrafish. a-d Zebrafish embryos at one-cell stage were microinjected with expression plasmids encoding WT (a), p.Arg79Cys GFAP (b and c), or p.Asp128Asn (d), and imaged at 30 hpf with transmission electron microscopy. Arrows and arrowhead indicate electron dense inclusions and a spherical structure with double-layered membranes, respectively. Scale bar = 2 μm
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