|
Fig. S16
Morphological abnormalities of ubi-pIDM-transgenic embryos after Dox induction. (A-B) Nine mutant lines had Dox-induced cardiovascular defects (A) and five lines had abnormal blood circulation and tail defects (B). Either wild-type (WT; left) or ubi-pIDM transgenic embryos (such as #6Tg; right) were treated with DMSO (control) or Dox (Dox-induction), and cardiovascular, tail and circulation phenotypes were documented at 48 hpf. The numbers on the lower right indicate phenotypical numbers out of the total (C) RNA in situ hybridization revealed that Dnajb5, proza, St6galnac1.2, and sps2 were expressed in the heart on the left column (lateral view), and the ventral view of the heart area is enlarged on the right column. Arrows point to the heart. Staged embryos are noted for dnajb5 at 36 hpf, for proza and st6galnac1.2 at 48 hpf, and for sps2 at 72 hpf.