ZFIN Image: Zabinyakov et al., 2017, Fig. 1

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Figure Caption

Fig. 1 QIAxcel system, Sanger sequencing and HRM analysis results. A) Gel image produced by QIAxcel system revealing wild type aldh7a1 sequence, as well as the heterozygous and homozygous 5bp deletion. The DNA sequences shown in the lower panel reveal the complement reverse sequences. The broken line indicates the nucleotides that were deleted by CRISPR/Cas9 in the homozygous fish. B) The HRM melting plots are displayed. The heterozygous sequence is identified by the double peak. The homozygous sequence is identified by a peak at 76.5 degrees Celsius, and the wild type sequence is identified by a peak at 77.5 degrees Celsius.

Acknowledgments

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