IMAGE

Fig. 5

ID
ZDB-IMAGE-180117-5
Source
Figures for Pena et al., 2017
Image
Figure Caption

Fig. 5 Early seizure onset and death following Lysine (Lys) treatment. Survival of aldh7a1−/− and wild-type (WT) siblings after 48 hr of exposure with Lysine 20 mM (A) started at days 6, 7, or 8 days postfertilization (dpf). Survival of aldh7a1−/− and WT siblings after 48 hr of treatment with Lys with or without daily Pyridoxine 10 mM (Pyr) treatments (B). Survival experiments were terminated at 20 dpf. Video analysis of 9-dpf larvae after 24 hr of treatment with Lys 20 mM, Pyr 10 mM, or both, showing significant increase in duration (C) and distance moved (D) in high-speed movements after light stimulus. Representative movement traces obtained from the Zebralab software at 9 dpf (24 hr after Lys treatment) showing the presence of larvae with hyperactivity and high-speed movements (red) (E). Blinded analysis of the same videos analyzed in (C–E) showing classification of each fish by seizure scores (S0, S1, S2, and S3), n = 8 larvae per group, except for mutant + Lys 20 mM where two fish died during the first 24 hr of treatment (F). Asterisks on top of the graph indicate statistical significance according to one-way ANOVA test [P = 0.0081 in (C) and P = 0.0060 in (D)] and asterisks comparing each pair of samples reflect Tukey’s post hoc pairwise tests (* P < 0.05 and ** P < 0.01). Error bars represent ± SD.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genetics