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Fig. S4
The three doses of GrnA rescue the hepatic outgrowth defect caused by miR-145 manipulation.
Liver morphology was determined by EGFP expression in Tg(fabp10:EGFP) embryos at 4 dpf (A) or in embryos injected with control mimic (B); miR-145 inhibitor (C); miR-145 mimic (D); miR-145 inhibitor with three doses of grnA MO 0.0625 ng/embryo (E), 0.125 ng/embryo (F), or 0.25 ng/embryo (G); and miR-145 mimic with three doses of grnA mRNA 0.1 ng/embryo (H), 0.2 ng/embryo (I), or 0.4 ng/embryo (J). (Scale bars, 100 μm; thirty embryos per experimental group were used and three independent replicates were performed).
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