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Fig. 3
Projections after a unilateral DiI injection into the olfactory bulb of an adult zebrafish shown at four telencephalic levels from anterior (A,A') to posterior (E,E'), with corresponding DAPI and fluorescent photomicrographs demonstrating tracing results. (A'') is a confocal blow-up at anterior levels detailing terminal fields and retrogradely labeled cells in the posterior zone of the dorsal telencephalon (Dp) and the dorsal nucleus of the ventral telencephalon (Vd). (C) shows section levels of (A) and (E). Sections (B) and (D) are the immediate caudal and rostral sections, respectively, and are not separately indicated. (D'') confocal photomicrograph shows Otpa staining in the preoptic region and the intermediate nucleus of the ventral telencephalon (Vi; insert). (F,F') are confocal blow ups of E' showing Di terminals and Otpa-positive cells in Vi. Stippled double arrows indicate midline. Abbreviations: acd: dorsal part of anterior commissure; Dm: medial zone of dorsal telencephalic area; Dl: lateral zone of dorsal telencephalic area; Dp: posterior zone of dorsal telencephalic area; OB: olfactory bulb; ENv: ventral entopeduncular nucleus; LI: hypothalamic lobus inferior; lot: lateral olfactory tract; mot: medial olfactory tract; PG: preglomerular complex; Pit: pituitary; Po: preoptic region; PPa: anterior parvocellular preoptic nucleus; TeO: optic tectum; TH: tuberal hypothalamus; TLa: torus lateralis; Vd: dorsal nucleus of ventral telencephalic area; SY: sulcus ypsiloniformis; Vi: intermediate nucleus of ventral telencephalon; Vp: posterior nucleus of ventral telencephalic area; Vv: ventral nucleus of ventral telencephalic area. I: olfactory nerve; II: optic nerve.