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Fig. 1
Pyriproxyfen is toxic at high doses.
(A) Percentage of survival of embryos treated from 2–4 cell stage with various pyriproxyfen concentrations ranging from 0,005 μg/ml to 1 μg/ml. The latest dose appears to be lethal with 100% of death after 5 days of development. Lower doses of pyriproxyfen (0,005; 0,01; 0,1 μg/ml) do not induce a severe decrease in the survival rate (N = 2 batches, n = 20 embryos per condition). (B) Higher doses of pyriproxyfen (10 and 100 μg/ml) are lethal as early as 2 dpf (N = 2, n = 20). (C) Representation of measurements performed on 7 dpf larvae to assess gross morphology effects. (D,E) Eye diameter and body length at 7 dpf of pyriproxyfen-treated embryos are not significantly different from vehicle (EtOH)- treated embryos (p > 0.05) (n = 10 per condition).