Fig. 1
Osmotic and heat shock lead to variable segment border defects.
Embryos were shocked at the 9 somite stage with a 840 mOsm solution (A-D) or 37°C embryo medium (E-H) for 45 minutes and allowed to develop until approximately 72 hpf. Arrowheads point to visible somite border defects. (A,E) Embryos exhibiting no defects. (B) The ventral portion of a somite border is curved. (C) The dorsal half of a somite border failed to form. (D) Multiple irregularly-formed somite boundaries. (F) A somite border is curved and the somite is narrow. (G) The ventral portion of two consecutive borders failed to reach the end of the somite. (H) Multiple irregularly-formed somite borders.