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Fig. 9
Escape latency of control and lesioned fish in response to a unilaterally directed squirt of water.
(A) Example trial showing the escape elicited by a squirt of colored water from a needle on the left side of the frame. The bend begins in the second frame (*) and the fish performs a rapid escape bend away from the stimulus in subsequent frames, with the peak initial bend at 10 ms (**). (B.) All of the data for the latency of escape response from lesioned (red) and unlesioned (blue) sides in controls and in the different ablation conditions. Box and whisker plots represent median as well as first and third quartiles. Outlier points are solid. Asterisks mark significant differences between responses on intact and lesioned sides (*p<0.05, ***p<0.001, corrected for multiple comparisons). Sham ablations and ablation of medial glycinergic cells near the M-cell, but not implicated in escapes, did not affect the latency. Killing the Mauthner cell led to a significant increase in latency. Cutting dendrite of the M-cell also led to a trend toward a longer latency, although it was not significantly different from the intact side. Ablation of the lateral FF glycinergic neurons led to a significant reduction in the latency to respond.