ZFIN Image: Koyama et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Specificity of laser targeting of inhibitory neurons.

(A) FF neurons were backfilled with Texas red in a transgenic line with neurons labeled with nuclear targeted GCaMP6s. (B1) Image prior to laser targeting showing the cell to be targeted marked by the white arrow head. (B2) GCaMP6s fluorescence from the targeted cell following laser illumination for ablation shows a massive increase in fluorescence, also visible in images of the targeted neuron in horizontal (C1) and cross section (C2) images taken after illumination. Note the bright green signal is evident only in the targeted cell and not in surrounding neurons next to and above it, showing the specificity of the targeting.

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