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Fig. S3
The lumen size of DA and PCV were not significantly changed in CA-4 treated zebrafish embryos. (A) Cross-section of Tg(kdrl:EGFP) control zebrafish embryos and embryos treated with CA-4 at different concentrations. (B) The statistics of DA and PCV lumen size, error bars indicate s.e.m. (20 embryos were analyzed for each group). (C) Whole-mount in situ hybridization analysis in 30 hpf control and CA-4 treated zebrafish embryos using flt4 (n=12) and dll4 (n=15) antisense probes.
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