|ZFIN ID: ZDB-IMAGE-171114-12|
Immunofluorescence staining of H3S10 in zebrafish embryos
(A-C'): Immunostaining of H3S10 in 48 hpf embryos. (D-F''): Transverse sections of forebrain (Top), midbrain (middle) and hindbrain (below) of different embryos immunostained for H3S10 (green).G: Quantification of H3S10 -positive cells. For each group, 6 embryos are scored (mean ± s.e.m,n=3, ***P<0.001, **P<0.01, *P<0.05, ns= not significant, Student’s unpaired t-test). e: eye, Fb: forebrain, Hb: hindbrain, Mb: midbrain,t: tectum.The dotted boxes mark the brain regions.
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