ZFIN Image: Kotani et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Suppression of mysterin-α leads to the failure of hatching and motor deficits in zebrafish.

(a) Examination of the knockdown efficiency in morphants. Treatment with a low (1.7 ng) or high (5.1 ng) dose MO targeting mysterin-α (Mst) confirmed dose-dependent suppression of mysterin gene splicing at 2 dpf, as determined by RT-PCR. The lower band represents the products of impaired splicing (arrow). (b) Hatching defects in mysterin morphants. Morphants injected with a high dose of MO exhibit a significant hatching failure at 3 dpf. (c) Escape response to tactile stimulation at 60 hpf. Injection of a MO targeting mysterin reduces the movement of larvae in a dose-dependent manner. (d) Quantification of the swimming speed (mm/s) measured in 6 control morphants, 7 low-dose morphants, and 5 high-dose morphants at 60 hpf. **P < 0.01; ***P < 0.001.

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Acknowledgments

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