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Fig. 2
miR-200 s repress expression of multiple GH/IGF axis genes during embryo development.
(A) Summary of the binding site of miR-200s in the GH/IGF axis genes predicted by Targetscan. (B) Expression of miR-141 and miR-429a in 24 hpf embryos injected with control or miRNA mimics. (C,D) Expression of GH/IGF axis genes in embryos injected with miR-141 and miR-429a mimic, respectively. (E,F) Whole mount in situ hybridization of GH in control and miR-141/429a mimic injected embryo at 48 hpf. (G) Protein expression of GH measured by western blot in 48 hpf embryo injected with miRNA mimics. (B–D) Error bars indicate mean ± SD, n = 3. Student’s t-test was used for statistical analysis (*p < 0.05).