ZFIN Image: Pauli et al., 2015, Fig. S1

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Figure Caption

Fig. S1

Specificity and toxicity of ASO-mediated RNA knockdown in zebrafish embryos.

A) oep ASO-mediated oep mRNA knockdown induces oep-specific phenotypes with high efficiency, while MALAT1 ASO-mediated MALAT1 lncRNA knockdown does not induce any visible, gene-specific phenotypes (3 dead (= black) embryos). B) ASOs induce toxicity at higher concentrations. The concentration at which a specific ASO is toxic for an embryo varies and is ASO sequence-dependent. In general, injection of > 200 pg of an ASO results in general toxicity. Shown are representative images of ASO-injected healthy (= no phenotype), deformed and dead embryos during mid-gastrulation (70% epiboly) and at 24 hpf.

Acknowledgments

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