ZFIN Image: Kirkwood et al., 2017, Fig. 3

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Figure Caption

Fig. 3

d-Tubocurarine and berbamine protect cochlear OHCs from gentamicin damage. Protection assay using cultures prepared from CD-1 mice at postnatal day 2 (P2). Cultures were stained with TRITC-phalloidin at the end of the experiment allowing visualization of the actin-rich stereocilia. (A) Control culture incubated for 48 h in the presence of medium alone. The sensory hair cell loss shown in (B) caused by 48 h incubation with 5 μM gentamicin can be prevented by co-incubation with 25 μM dTC (C) or 20 μM berbamine (D). Cells incubated in 50 μM dTC in the absence of gentamicin were not damaged (E), whereas 30 μM berbamine resulted in widespread loss of IHCs, OHCs and other cell types (F). Scale bar = 50 μm.

Acknowledgments

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