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Fig. S2
Ectopic Tbx16 is ubiquitous, labile and peaks at 4 hours post-heat shock. In control (A-C) and HS:tbx16 (D-F) embryos, immunofluorescence shows that Tbx16 protein levels have increased by 2 hours post-heat shock (A, D) with a peak at 4 hours post-heat shock (B, E), and that levels of Tbx16 have largely returned to normal by 6 hours post-heat shock (C, F). Inset in D shows ectopic Tbx16 in the notochord and neural tube (marked by arrowheads) when compared to the control inset in A (both dorsal views of the tailbud); arrows in E, F highlight the telencephalon, which retains ectopic Tbx16.
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