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Fig. 2
Iodixanol is live specimen compatible.
(a) Iodixanol does not affect growth and cell death levels in cultured HeLa cells. Left: Representative low-resolution images of the constitutively expressed nuclear marker H2B-mCherry at indicated incubation times and media conditions. Scale bar = 50 µm; Right: Quantification of cell numbers (number of nuclei) and dead cells (DRAQ7 positive nuclei) at the indicated time points and Iodixanol concentrations. Iodixanol was applied 24 hr post seeding and measurements were normalized to the 24 hr time point in order to compensate fluctuations in plating density. n = 3; See Figure 2—figure supplement 1 for a complete data representation. (b) Iodixanol does not affect developmental growth or survival of dechorionated zebrafish embryos. Left: Representative images of developing embryos at the indicated time points (hpf = hours post fertilization) and media conditions. N = 5; Scale bars = 100 µm at 2 and 16 hpf, 500 µm at 48 and 72 hpf. Right: Quantification of body length and survival rate at the indicated time points and media conditions. The initial drop in the survival curves is an effect of dechorionation. N = 30; (c) Iodixanol does not affect regeneration of the planarian head or body proportions. Left: Representative images of regenerating Dendrocoelum lacteum amputation fragments at the indicated time points (dpa = days post amputation) and under the indicated media conditions. Anterior is always up, Scale bar = 500 µm; Right: Quantification of length/width ratio and projected area at the indicated time points and media conditions. Measurements were normalized to the 0 time point in order to compensate initial size differences between tissue pieces. N = 3; (a–c) Error bars represent S.E.M. p>0.05 in all cases: (a) one way ANOVA (b, c) paired t-test.