IMAGE

Fig. S4

ID
ZDB-IMAGE-170921-45
Source
Figures for Gallagher et al., 2017
Image
Figure Caption

Fig. S4

Venus reporter transcripts accumulate in pnrc2- and upf1-deficient embryos, but Venus protein expression appears normal. Embryos carrying the her1:her1-Venusbk15 transgenic clock reporter were injected with a moderate pnrc2 sbMO dose (6 ng) or co-injected with low pnrc2 and upf1 sbMO doses (2 ng and 0.25 ng, respectively) to detect Venus transcripts (A-C) and Venus protein (D-F) at mid-segmentation stages. Representative embryos are shown in A (n=12), B (n=8), C (n=9), D (n=36), E (n=24), and F (n=27). Venus immunofluorescence panels (D-F) are at slightly higher magnification than Venus in situ panels (A-C). sbMO = splice-blocking MO; scale bar = 50 um (D-F).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.