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Fig. 2

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ZDB-IMAGE-170921-37
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Figures for Gallagher et al., 2017
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Fig. 2

tor mutant embryos are rescued by injection of pnrc2 mRNA and phenocopied by frame-shifting mutation of the pnrc2 locus. Injection of 100 pg pnrc2 mRNA has no effect in wild-type sibling embryos (n=15/15 non-mutant siblings) (A, A’ vs B, B’) but restores striped her1 expression in tor mutant embryos (n=10/10 mutants) (C, C’ vs D, D’; Table 2). In these experiments, rescued torb644 mutant embryos were distinguished from wild-type siblings by lack of expression of pou3f1, a gene located in the torb644 deficiency interval. Asterisks (*) mark the neural pou3f1 expression domain. Arrows mark the her1 expression domain (A-D), magnified in dorsal view to the right of each embryo (A’-D’). Using CRISPR-based mutagenesis, we induced a 17 bp deletion within the pnrc2 coding sequence, creating an early frameshift allele, designated pnrc2oz22 (E). Predicted mutant protein sequence is based on sequenced genomic DNA (E). The pnrc2oz22 allele fails to complement the her1 accumulation phenotype of the torb644 deletion allele (n=4/15 embryos from a heterozygote intercross with tor-like her1 accumulation) (F, G). Similar to torb644 mutants, pnrc2oz22 mutant embryos lack a striped her1 expression pattern (H, H').

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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.