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Fig. 7
Reduced cx43 prior to joint formation increases the frequency of evx1-positive rays. A: Whole-mount ISH shows decreased cx43 expression and increased evx1 expression in the Tg(hsp70:miR-133a1)pd47 line at the time of joint initiation (87 hpa) in a1(+) (transgene-positive) vs. a1(-) (nontransgenic). Bottom panels in A show evx1-positive fin rays across entire fins. B: A higher percentage of fin rays in a1(+) transgenic fins showed expression of evx1 (n = 9 fins) compared to the a1(-) nontransgenic sibling (n = 9 fins). Both sp(+) and sp(-) fish were similarly treated for heat shock. Statistically significant differences were determined by the Student's t-test, where P < 0.01 (**). Error bars represent the standard deviation.