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Fig. 6
Inhibition of calcineurin (FK506 treatment) influences joint formation and regenerate length. A,B: Calcein staining detects bone matrix and reveals joint failure and increased regenerate length in FK506-treated wild-type fish compared to control DMSO-treated wild-type fish. Panels on the left show fluorescence; panels on the right show fluorescence plus bright-field to better illustrate the end of the regenerating fin (the dotted line indicates the amputation plane). Arrowheads point to joints. Double-headed arrows identify regenerate length. C: Regenerate length is significantly increased FK506-treated (n = 18) vs. DMSO-treated (n = 18, negative control) fish. Statistically significant differences were determined by the Student's t-test, where P < 0.001 (***). Error bars represent the standard deviation.