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Fig. 7
The absence of Gp91 results in increased bacterial burden and neutrophil persistence during subcutaneous infections.
(A) Bacterial burden over time after subcutaneous injection of B. cenocepacia K56-2 in control (black circles) and gp91 knockdown embryos (open red circles). Average of two independent experiments. (B) Bacterial burden over time after subcutaneous injection of B. stabilis LMG14294 in control (black circles) and gp91 knockdown embryos (open red circles). Average of three independent experiments. (C) Fluorescent overlay images of the injected area of a representative mpx:GFP control MO and gp91 knockdown embryo (neutrophils in green) in time after subcutaneous injection with B. stabilis (red). Inset shows bacterial load at ~20 hpi. % at 22 and 21 hpi indicates percentage of control and gp91 knockdown embryos that show reduced neutrophil numbers (86.8%), and persistent neutrophil infiltration (71.3%), respectively, at the infection site. Scale bar, 100 μm. (A,B) * p ≤ 0.05; **** p ≤ 0.0001; ns: non-significant. See materials and methods for statistical tests.