ZFIN Image: Zhang et al., 2017, Fig. 1

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Figure Caption

Fig. 1

eIF3i is expressed in zebrafish and mammalian endothelial cells. A. eIF3i is expressed in endothelial cells of 24 hpf zebrafish embryos as determined by WISH. The black arrowheads point to the dorsal aorta and intersegment vessels of embryos. B. Human umbilical vein endothelial cells (HUVECs) expressed eIF3i. The red channel represents eIF3i, and the blue channel represents the cell nucleus. Scale bars, 10μm. C. eIF3i siRNAs significantly inhibited eIF3i protein level. GAPDH was used as a loading control. Fold changes are compared with mock and presented below each blot. D. eIF3i siRNAs significantly inhibited eIF3i mRNA level. (n=3).***p<0.001. E. HCM increased the expression of eIF3i in HUVECs. Tubulin was used as a loading control. Fold changes are compared with BM and presented below each blot. BM, basic medium; CM, B16 cell non-hypoxic conditional medium; HCM, B16 cell hypoxic conditional medium.

Figure Data

Expression / Labeling details

Acknowledgments

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