IMAGE

Fig. 4

ID
ZDB-IMAGE-170516-36
Source
Figures for Goto et al., 2017
Image
Figure Caption

Fig. 4

FGF regulates tailbud cell displacement through tbx16 regulation. (A-E) Time-lapse images of photoconverted tailbud cells of NLS-kikume mRNA-injected wild-type cells (Aa-d′,Ca-d′), HS:tbx16 (Ba-d′,Da-d′) and HS:cafgfr1 (Ea-d′) embryos at 12-somite stage and onward, as indicated. DMSO-treated wild-type cells were able to move rapidly from the progenitor zone (Aa-d′), whereas SU5402-treated wild-type cells remained within the tailbud (Ca-d′). Activation of tbx16 rescues cell displacement and speed in the presence of SU5402 (Da-d′). Cells with activated FGF signaling also enter the maturation zone similarly to wild-type embryos (Ea-d′ versus Aa-d′), but not as rapidly as tbx16-overexpressing cells (Ba-d′). White arrowheads point to cells entering the maturation zone. (F,G) Tracking of photoconverted cells as shown in trajectories (Fa-e) and displacement vector (Ga-e). (H-J) The mean displacement, mean track speed and mean track straightness of photoconverted cells. The mean displacement and the speed decrease in SU5402-treated wild-type embryos compared with vehicle-treated wild-type embryos (H versus I). tbx16 overexpression rescues the displacement and speed in SU5402-treated embryos. Track straightness did not differ significantly among the treatment groups (J). *P<0.05, unpaired two-way t-tests.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development