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Fig. 6
CHEST cells encourage differentiation of HSPCs in culture.
(A) Composite May-Grünwald Giemsa images of hematopoietic cells isolated from the lymphoid fraction in Fig. 5 (1000x, scale bar is 10 μm). (B) Composite May-Grünwald Giemsa images of hematopoietic cells isolated from the precursor fraction in Fig. 5 (1000x, scale bar is 10 μm). (C) RT-PCR of leukocyte- (cd45), progenitor- (cmyb), erythromyeloid- (cd41, mpx, band3, and gata1), B cell- (pax5 and igm), and T cell- (lck) specific transcripts isolated from the cultures as in A and B. Gel images have been cropped for clarity of presentation.
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