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Fig. 4
CHEST cells support hematopoietic expansion.
(A) WKM from adult zebrafish was plated on CHEST cells and enumerated at various times (black circles; n = 8), plated in culture with no stromal under layer (black squares; n = 6), or plated with no CHEST cells in CHEST cell supernatant media (black triangles; n = 3). Error bars denote standard error of the mean. * denotes p < 0.005 and ** denotes p < 0.0005 when comparing cells grown on CHEST cells to either of the two other samples. (B) Composite May-Grünwald Giemsa images of hematopoietic cells isolated from the cultures (1000x, scale bar is 10 μm).
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