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Fig. S3
Glial specification is severely defective in baz1 mutants similar to other sox10 mutant alleles.
A-L) sox10 expression at 48 hpf. Columns show sox10-expressing cells in head (A, E, I), lateral pathway of trunk (B, F, J), medial pathway of trunk (C, G, K) and tail (D, H, L) respectively of 48 hpf WT (A-D), baz1 (E-H) and m618 (I-L) mutant embryos. Glia of cranial ganglia and Schwann cell precursors on cranial and PLL nerve (white arrowheads) are prominent in WT, but highly reduced or nearly absent in baz1 and m618 mutants respectively. Cells of DRGs (white asterisk) form a prominent segmentally reiterated pattern on the medial pathway of trunk and tail of WT, but are reduced in sox10 mutants; note that in tail, where cells are developmentally younger, the DRG patterns are more similar. Xanthoblasts (black asterisk) are prominent on lateral pathway of WTs, but reduced and absent in baz1 and m618 mutants respectively. M,N,P,Q) foxd3 expression at 48 hpf. Expression in Schwann cell precursors associated with spinal nerves forms segmentally reiterated pattern readily seen in WT (M), but numbers of cells are much reduced in baz1 (P). foxd3-expressing Schwann cell precursors (white arrowhead) on PLLn are prominent in WT (N), but highly reduced in number in baz1 at 48 hpf (Q), where they are usually seen only anteriorly. O,R) Supernumerary neuromasts in baz1 mutants. Whole mount In situ hybridisation with isl1 probe in 48 hpf WT (O) and baz1 mutant (R). Note supernumerary neuromasts (black arrowheads) in baz1 mutant. Photos all from single, typical individual of each genotype, after PTU treatment.