Fig. S1

Fig. S1

An Epigenetic Signature for Putative OR Enhancers, Related to Figure 1

(A) DHS-seq and ChIP-seq tracks at protocadherin α enhancer HS4.

(B) Analysis pipeline for generating OR enhancer candidates (column 1) and MOE enhancer candidates (column 2). Analysis for OR enhancers is restricted to genomic coordinates of the 52 OR clusters. Intergenic peaks were selected to be at a distance of 5kB or more from a Refseq gene or OR promoter. First three rows list the number of intergenic peaks from DHS-seq and H3K27ac and H3K24me1 ChIP-seq data. Fourth row lists the number of DHS peaks that intersected either H3K27ac or H3K4me1 peaks. Fifth row subtracts DHS peaks that intersected either H3K27ac or H3K4me1 SICER peaks from cerebellum ChIP-seq data set. These are the sequences referred to as “OR enhancers” or “MOE enhancers,” respectively, in the text. Sixth row is number of OR enhancers or MOE enhancers that intersect an H3K79me3 peak.

(C) Representative zebrafish embryos from oocyte injections of 11 candidate OR enhancers and H enhancer. GFP expression in the olfactory epithelium is indicated. See Table S2 for enhancer coordinates.

(D) Representative images of zebrafish OSNs expressing GFP at 48hpf from 6 OR enhancer transgene injections.

(E and F) Heatmaps of H3K79me3 and H3K4me1, respectively, RPKM over OR enhancer candidates. y axis is OR enhancer candidates ordered by principal component 1 of each chromatin modification matrix. x axis is centered at DHS peak. Red ticks indicate H, P, and the 11 sequences that drove GFP expression in zebrafish OSNs.