ZFIN Image: Boke et al., 2016, Fig. 1

Image

Figure Caption

Fig. 1

A Balbiani Body Is a Non-Membrane-Bound Compartment Packed with Membranous Organelles

(A) Phase contrast image of a stage I Xenopus laevis oocyte. Bb, Balbiani body; N, nucleus or germinal vesicle.

(B) Balbiani body immobilized in perfusion chambers. 2 M NaCl (first panel) or 95°C 50 mM HEPES, 100 mM KCl (pH 7.6) buffer (second panel) was perfused into the chambers.

(C) Thin-section electron microscope (EM) images of isolated Balbiani bodies from stage I Xenopus oocytes. Mitochondria (dark spots), RNP particles (green arrow head), and Golgi stacks (yellow arrow head) are clearly visible.

(D) Stage I oocytes were incubated in 10 μM Thioflavin T in 1× MMR for 10 min and washed twice with 1× MMR.

See also Figure S1, Table S1, and Movie S1.

Acknowledgments

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Reprinted from Cell, 166, Boke, E., Ruer, M., Wühr, M., Coughlin, M., Lemaitre, R., Gygi, S.P., Alberti, S., Drechsel, D., Hyman, A.A., Mitchison, T.J., Amyloid-like Self-Assembly of a Cellular Compartment, 637-50, Copyright (2016) with permission from Elsevier. Full text @ Cell