ZFIN Image: Ziegler et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Identification of a Thymosin 4 ortholog in zebrafish. (a) Amino acid sequence alignment of zebrafish beta Thymosin and beta Thymosin-like andmouse and humanThymosin 4. The yellow box indicates the consensus actin bindingmotif, indicating a closer degree of homology of zebrafish betaThymosin-like to themammalianThymosins than betaThymosin. (b)The comparison of the genomic localization of beta Thymosin and beta Thymosin-like in zebrafish, in relation to the murine and human localization, shows a closer proximity of beta Thymosin-like than beta Thymosin to the mammalian Thymosin 4. (c) Vector used to integrate pEGFP-N1-tmsb-like in zebrafish with indicated binding site of tmsb-like morpholinos (tmsb-like-MO) used to knockdown tmsb-like in zebrafish. (d) Treatment of pEGFP-N1- tmsb-like expressing zebrafish embryos with tmsb-like-MO demonstrates high knockdown efficiency.

Acknowledgments

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