ZFIN Image: Bielczyk-Maczyńska et al., 2015, Fig. S9

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Figure Caption

Fig. S9

The number of thrombocytes and neutrophils is unaffected in nol9^{sa1022/sa1022} mutants.

(A) Representative maximum projection confocal images showing thrombocytes (GFP^bright, arrow) and HSPCs (GFP^dim, arrowhead) in the CHT of Tg(cd41:EGFP) larvae at 96 hpf. Larvae are oriented with anterior to the left and dorsal to the top. (B) The number of GFP^bright thrombocytes observed in the CHT region of nol9^{sa1022/sa1022} mutants (n = 20) and their wt siblings (n = 51) at 96 hpf. Data are represented as the mean +/- SEM; Student’s t-test, p>0.05. (C) Images of the CHT region of 72 hpf larvae stained with Sudan Black B. Stained neutrophils are marked by an arrow. (D) The average number of Sudan Black B-stained neutrophils in the CHT region of 72 hpf larvae, depending on their genotype (nol9^+/+ n = 32, nol9^+/sa1022 n = 32 and nol9^{sa1022/sa1022} n = 28). Data are represented as the mean +/- SEM; One-way ANOVA, ns–not significant.

Acknowledgments

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