IMAGE

Fig. 4

ID
ZDB-IMAGE-170125-6
Source
Figures for Cepero Malo et al., 2017
Image
Figure Caption

Fig. 4

Anillin-eGFP in the CMZ marks transit-amplifying progenitors entering restricted neuronal lineages.

Top panel: view of the retina in the transversal plane (eye position 90° relative to the viewer). The blue line across the retina corresponds to the sagittal view (optical z-section) in the top panel of Fig 3. (A-C) and (E-I) represent two optical sections from confocal z-stacks through the retina of a 48 hpf (A-C) and 3 dpf (E-I) zebrafish taken in the transversal plane. The squared dotted bracket in (A) and (E) delineates the CMZ domains. The squared bracket in (A-C) and (E-H) delineates cells within the CMZ that are both Anillin-eGFP (green) and Rx2 immunoreactive (blue). (D) As the stem cell niche develops, the percentage of Rx2 immunoreative cells that are also Anillin-eGFP positive in the CMZ domain decreases over time from 20% to 11,4%. The arrows in (F-I) point at two dividing, Anillin-eGFP (green) positive cells and at their nuclei (I). (F,G) One of the two cells is also (ptf1a)dsRed positive and non-apically located. Ph, photoreceptors; ac, amacrine cells; hc, horizontal cells; mg, Müller glial cell. ONL, outer nuclear layer; INL, inner nuclear layer; ONL, outer nuclear layer.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One