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Fig. S10
Crest cells are not affected in col4a6 mutants.
Immunostaining of 5-dpf wild-type (A, Da-Dc) and col4a6 mutant (B, Ea-Ec) larvae with anti-Vglut1 (A, B, Da, Dc, Ea, Ec) and anti-Pvalb7 (A, B, Db, Dc, Eb, Ec) antibodies. Higher-magnification images of boxes D and E in A (Da-Dc, Ea-Ec). The number of crest cells in each larva is indicated (C). The number of crest cell is not significantly different between wild-type and col4a6 mutants (n.s., Student’s t-tests p = 0.504). Scale bars: 50 μm in A (applied to B), 20 μm in Da (applied to Db-Gc).
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