IMAGE

Fig. 4

ID
ZDB-IMAGE-161121-10
Source
Figures for Hilario et al., 2009
Image
Figure Caption

Fig. 4

plexin B1-A and plexin B1-B morpholino injection phenotypes and co-injections with sema5A. (A) Injection of 6 ng plexin B1-A MO shows an increase in branching CaP axons compared to control MO injected embryos. (B) Co-injection of 3 ng plexin B1-A MO and sema5A MO has little or no difference with injection of each MO by itself at 3 ng. (C) Injection 6 ng plexin B1-B MO results in a delay in CaP axon extension into the ventral myotome compared control MO injected embryos. (D) Co-injection of 3 ng of plexin B1-B MO and sema5A MO displayed no delay of CaP axon extension phenotype. Data quantified from 3 ng sema5A MO injected embryos (n = 2020 axons, 101 embryos), 6 ng plexin B1-A MO injected embryos (n = 1040 axons, 52 embryos), 3 ng plexin B1-A MO injected embryos (n = 940 axons, 47 embryos), 3 ng sema5A MO + 3 ng plexin B1-A MO injected embryos (n = 2140 axons, 107 embryos), 6 ng plxnB1B MO injected embryos (n = 1960 axons, 98 embryos), 3 ng plexin B1-B MO injected embryos (n = 1080 axons, 54 embryos), 3 ng sema5A MO + 3 ng plexin B1-B MO injected embryos (n = 1180 axons, 59 embryos). Error bars represent confidence interval for proportions at 95% confidence. Asterisks indicate significant difference between control MO injected and plexin B1-A or plexin B1-B MO injected with p < 0.001 using one-way ANOVA.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 326(1), Hilario, J., Rodino-Klapac, L.R., Wang, C., and Beattie, C.E., Semaphorin 5A is a bifunctional axon guidance cue for axial motoneurons in vivo, 190-200, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.