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Fig. S3
Defect in caudal migration is not due to a delay in cell movements in Tg(isl1:cdh2ΔEC-mCherry)vc25 transgenic embryos.
(A-B) Whole-mount immunocytochemistry showing dorsal view of wild-type Tg(isl1:GFP) (A) and Tg(isl1:cdh2ΔEC-mCherry)vc25 transgenic embryos (B) at 48 hpf. Embryos are labeled with α-GFP (green) (A and B) and α-mCherry (red) (B) antibodies. (A) Wild-type Tg(isl1:GFP) embryos with FBMNs fully migrated into r6. (B) There is a dramatic defect in caudal migration of FBMNs in homozygous Tg(isl1:cdh2ΔEC-mCherry)vc25 embryos at 48 hpf, when FBMN migration is normally complete. (C) Histogram indicates the percent of FBMNS at 48 hpf that failed to migrate (r4), migrated partially (r5), or migrated fully (r6). Each histogram corresponds to the genetic condition in the image to its left and numbers indicate the number of FBMNs counted. White asterisk denotes r6-derived PLL efferent neurons, which differ from r4-derived FBMN populations.