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Fig. 7
Bioenergetic analysis of Opa1 morphants.
Oxygen consumption rates (OCR) were measured in 24, 48, and 72 hpf morphants (mean +/- SEM, n = 5–10). A. Opa1 morphant basal respiration was significantly decreased compared to MMC morphants at 24 and 72 hpf (p = 0.004 (a) and p = 0.0007 (b), respectively). Maximal respiratory capacity and proton leak were not different between groups at any time point. ATP turnover was significantly decreased for Opa1 morphants at 24 and 48 hpf (p = 0.03 (c) and p = 0.04 (d), respectively). B. The proportion of total basal respiration due to non-mitochondrial respiration, ATP turnover and proton leak were similar between Opa1 and MMC morphants. C. Respiratory control ratio was significantly greater for Opa1 morphants than MMC at 24 and 72 hpf (asterisks, p<0.03). P values were determined by Student′s 2-tailed t-test.