IMAGE

Fig. 3

ID
ZDB-IMAGE-161020-1
Source
Figures for Ransom et al., 1996
Image
Figure Caption

Fig. 3

Mutations in the genes weißherbst (weh), zinfandel (zin) and sauternes (sau) result in embryos with hypochromic blood and decreasing blood cell counts. (A,C,E,G,I) Isolated blood cells from wild-type and mutant larvae stained by the Wright-Giemsa method. (B,D,F,H,J) O-dianisidine staining for hemoglobin (arrows) in wildtype and mutant embryos. (A) Blood cells from 2-day old wild-type embryos have the normal morphology of late erythroblasts. (B) Wild type embryos express large amounts of hemoglobin in their differentiating red blood cells. (C) Blood cells from 2-day old wehtp85c mutant embryos are larger with open nuclei more typical of early proerythroblasts. (D) 2-day old wehtp85c mutant embryos have approximately 50% of the normal number of blood cells which express much lower levels of hemoglobin compared to wild-type blood cells. (E) Blood cells of 2-day old zinte207 mutant embryos are large but are more differentiated than the blood cells of weh mutant embryos. (F) 2-day old zinte207 heterozygotes (shown here) and homozygotes both lack detectable hemoglobin. (G) 3-day old wildtype embryos have mature erythrocytes with condensed nuclei and red staining hemoglobin. (H) A wild-type, 3-day old, embryo has large amounts of blood expressing hemoglobin. (I) Blood cells of 3- day old sauy121a mutant embryos are relatively normal in morphology, but they are reduced in number to between 25% and 50% of normal levels with a few large basophilic cells. (J) 3-day old sauy121a mutant embryos have fewer blood cells and thus a reduced total amount of hemoglobin expression. The scale bar in panel (A) is equal to 20 microns for panels (A,C,E,G,I).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development