ZFIN Image: Lalonde et al., 2016, Fig. 8

Image

Figure Caption

Fig. 8

Epi site 3 required for epithelial reporter expression in Tg(2Pand1:eGFP). (A-G) 3 d.p.f. Tg(2Pand1:eGFP) epi site 3 transgenic reporter line. Ectodermal expression is completely lost with the removal of the epi site 3 23 b.p. fragment (A-G). Median (E-G) and pectoral fin fold (B-D) display reporter expression in the mesenchymal cells identical to Tg(2PΔepi:eGFP) transgenic line. Note the higher number of eGFP-positive cells in the posterior half of the pectoral fin fold (B, C). Brightfield (B, E), fluorescence (A, C, F), and brightfield/fluorescence merged images (D, G). ED, Endoskeletal Disc; FF, Fin Fold; A, Anterior; P, Posterior; D, Dorsal; V, Ventral. Scale bars: 200 µm in A; 50 µm in B-G.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 417(1), Lalonde, R.L., Moses, D., Zhang, J., Cornell, N., Ekker, M., Akimenko, M.A., Differential actinodin1 regulation in zebrafish and mouse appendages, 91-103, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.