|
Fig. S3
Figure S3: Ectopic expression of Med10 rescues png mutant embryos, whereas knockdown of zmed10 phenocopies the png mutant phenotype. (A-D) Ectopic expression of wild-type med10 RNA can rescue the heart phenotype of a significant proportion of png mutant embryos (84.5 ± 4.2%, n = 85, 3 independent experiments) (B, C), whereas KCl control-injection has no effect (A, C). (D) Hematoxylin/Eosin staining of sagittal histological sections of a med10 RNA-injected png mutant heart at 72 hpf. Endocardial cushion development (black arrow) in png mutant hearts is reconstituted. (E-G) Knockdown of zmed10 by injection of Morpholino-modified antisense oligonucleotides (MO-med10) phenocopies the png mutant phenotype (MO1-med10: 98.37% ± 0.45%, n = 240, 3 independent experiments; MO2-med10: 95.53% ± 0.68%, n = 185, 3 independent experiments) (E, G), whereas injection of the same amount of standard control Morpholino-modified antisense oligonucleotides (MO-control) does not impact on AV canal development (F, G).