ZFIN Image: Talbot et al., 2016, Fig. 4

Image

Figure Caption

Fig. 4

fras1 and itga8 are expressed in adjacent facial tissues. (A–D) Colorimetric in situ hybridization, developed using NBT/BCIP on wild-type sections, showing fras1 expression in pharyngeal and ectodermal epithelia (A, B) and itga8 expression in mesenchyme (C and D) at 36 hpf (A and C) and 72 hpf (B andD). (E) Color-coded diagram of lateral and transverse sections from a wild-type 60 hpf embryo, showing the first two pharyngeal arches (green), ectoderm (orange), cartilage (blue), and endoderm (red). (F and G) Fluorescent RNA in situ for fras1, itga8, and col2a1 expression in a 60 hpf transverse section of wild-type embryos at (F) low and (G) higher magnification. All scale bars: 50 µm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 416(1), Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B., Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction, 136-48, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.