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Fig. 5
Expression of rrm2b gene in embryos exposed to DNA damage reagents. Total RNA was isolated for real-time PCR analysis and transcriptional expression of β-actin was used as the reference. The ratio of p53R2 to β-actin for untreated embryos in each group was normalized to 1 and data for treated embryos represent relative values of untreated embryos. Data are expressed as means ± SD from three independent experiments. (A) Embryos at 24 hpf were exposed to 250 nM CPT or 550 nM MMS for indicated time intervals. (B) Embryos at 24 hpf were exposed to indicated concentrations of CPT or MMS for 6 h.
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Reprinted from Gene, 475(1), Shang, H., Li, Q., Feng, G., and Cui, Z., Molecular analysis and functions of p53R2 in zebrafish, 30-38, Copyright (2011) with permission from Elsevier. Full text @ Gene