|
Fig. 4
Apical Par proteins mediate the effects of miR-219 function on Hh signaling. (A-C) In situ RNA hybridization to detect ptch2 expression in 3dpf control, pard3 TP MO-injected and prkci TP MO-injected embryos. Dashed circles outline the spinal cord and brackets mark ptch2 expression domain. (D) Graph showing relative ptch2 and gli1 RNA levels measured by qPCR. Data represent the mean±s.e.m. (n=3-5 biological replicates, consisting of 15-20 pooled embryos). Significance calculated using an unpaired t-test. (E-I) In situ RNA hybridization reveals reduced ptch2 levels in 2dpf and 3dpf prkci mutant embryos and prkci mutant embryos injected with miR-219 MO. (J) Graph showing relative ptch2 and gli1 expression levels measured by qPCR. Data represent the mean±s.e.m. (n=3 biological replicates consisting of 15-20 pooled embryos, each). Significance calculated using an unpaired t-test.