IMAGE

Fig. 1

ID
ZDB-IMAGE-160805-9
Source
Figures for Wolman et al., 2015
Image
Figure Caption

Fig. 1

Genetic Screen Identifies Mutations Affecting Acoustic Startle Habituation

(A) Schematic of acoustic startle habituation assay. Larvae are exposed to 10 non-habituating acoustic stimuli, delivered at 20 s interstimulus intervals (ISI), and then 30 habituating stimuli at a 1 s ISI.

(B) Mean acoustic startle habituation percentage calculated by comparing the average frequency of startle responsiveness of an individual to stimuli 1-10 and stimuli 31-40 (Wolman et al., 2011). Behaviorally defined wild-type siblings shown in white bars, mutants in gray bars.

(C) Estimated truncated PCXAp171 protein in information overloadp171 mutants due to Y1163X mutation. BC, biotin carboxylase; CT, carboxyl transferase; PT, pyruvate carboxylase tetramerization; BCCP, biotin-carboxy carrier protein.

(D) Estimated truncated PAPP-AAp170 protein product in unfilteredp170 mutants due to Q322X mutation. LG, laminin G-like module; LNR, Lin-12/Notch repeats, MPD, metzincin proteolytic domain containing zinc-binding consensus sequence (Zn) and Met-turn motif (M); CCP, complement control protein modules 1-5.

E) pappaap170 larvae injected with increasing doses of wild-type pappaa mRNA show improved habituation at 5 dpf. *p < 0.01, **p < 0.001, ANOVA with Bonferonni correction versus wild-type sibling (B) or uninjected pappaap170 (E) larvae. N = number of larvae shown within or below each bar. Error bars indicate SEM.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron