ZFIN Image: Hartsock et al., 2014, Fig. 8

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Fig. 8

The lens is required for Stages II and III hyaloid maturation and maintenance. All images are stills from time-lapse movies from severe mab21l2^au10; fli1a:GFP mutants. (A) Hyaloid precursor cell recruitment is delayed by ~2 h in mab21l2^au10 mutants but recruitment is otherwise normal. (B) Hyaloid loop formation occurs in the absence of a lens. (C) Stage II formation of a branched hyaloid network is disrupted in mab21l2^au10 mutants. Hyaloid cells appear disorganized and dynamic, having not coalesced into obvious vessels. (D) The hyaloid in mab21l2^au10 mutants still makes contact anteriorly with the annular vessel. (E) Microangiography demonstrates that at 3d pf, the mab21l2^au10 mutant hyaloid is not enclosed anteriorly, and rhodamine (red) fills the retina. hh:mm. Beige dashed line in A, B and D indicate outlines of the retina. All scale bars=50 µm. D: Dorsal, V: Ventral, N: Nasal, T: Temporal, L_a: Lens anterior, L_p: Lens posterior.

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Acknowledgments

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Reprinted from Developmental Biology, 394(2), Hartsock, A., Lee, C., Arnold, V., Gross, J.M., In vivo analysis of Hyaloid vasculature morphogenesis in zebrafish: A role for the lens in maturation and maintenance of the Hyaloid, 327-39, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.