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Fig. S1
Tagged Nodals show similar activity compared to their untagged counterparts.
(A) Induction of ntl in embryos overexpressing Nodal or Nodal fusions. Five picogram aliquots of RNA was injected into one-cell stage wild-type embryos and ntl transcript expression was examined at 50% epiboly. Animal pole views of embryos showing endogenous ntl expression (I), and mild (II) or massive (III and IV) expansion of the ntl expression domain. Embryos were assessed and counted accordingly. Percentages for each class are shown in the histogram. (C) Induction of gsc in embryos overexpressing Nodal or Nodal fusions. Animal pole views of embryos showing endogenous gsc expression (I), mild expansion (II) or massive expansion (III and IV) of gsc expression domains. (C) Five picogram aliquots of RNA encoding Nodal or Nodal fusions were injected into one-cell at the 128-cell stage with a lineage tracer (Biotin-Dextran, brown color staining). Range of signaling was examined by detecting ntl transcription (blue/purple color staining). Scale bars represent 100 µm.