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Fig. 4

ID
ZDB-IMAGE-160606-20
Source
Figures for Johnson et al., 2016
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Figure Caption

Fig. 4

Metronidazole (Mtz) treatment induces radial glial cell death during late neurogenesis. (A-F) Gross morphology of transgenic gfap:nfsb-mCherrysc059 embryos following treatment. Enlargements comparing brain hemorrhaging compared with control in insets. Scale bar = 100 µm. (G, H) Lateral views of cell death within the spinal cord visualized by immunolabeling with antiactivated caspase-3. Scale bar = 20 µm. (I-M) Images of live gfap:nfsb-mCherrysc129 embryos at 28 and 38 hpf expressing secreted Annexin-V YFP labeling. (M) Example of NTR-mCherry+ cell with radial glial morphology expressing Annexin-V (YFP) labeling undergoing apoptosis over the course of 72 min.

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