IMAGE

Fig. S2

ID
ZDB-IMAGE-160524-11
Source
Figures for Eno et al., 2016
Image
Figure Caption

Fig. S2

Blastodisc height and chorion expansion in wild-type and aura mutant embryos. (A,B) Blastodisc height in wild-type (n=39) and aura mutant embryos (n=42) (A). Height (orange lines, B) was measured as a percentage of the whole embryo length (red lines) at 30 mpf. Average blastodisc height in mutants is mildly yet statistically significantly reduced compared to wild-type (t-test, p-value=0.020). (C,D) Average chorion expansion in mutants is mildly yet statistically significantly reduced compared to wild-type in two of three measured time points (t-test type, p- values: 5 mpf: 0.010, 10 mpf: 0.16; 30 mpf: 0.0099). (C). Chorion lifting was measured by subtracting the area of the embryo (orange circle in D) from the area of the chorion (red circle in D). A fraction of aura mutant embryos (60%, n=1096) exhibit chorion fragility in which it separates as sublayers (E, arrows). Although we can not rule out other interpretations, this chorion fragility phenotype that can be interpreted as a consequence of reduced chorionic protein modification of chorion sublayers (Ulrich, 1969; Hart and Collins, 1991; Selman et al., 1993) due to decreased CG release in aura mutants. Embryos were a random collection from 3 females per genotype. Bars represent standard error values. Brackets reflect degree of statistical significance, with p-values < 0.01 (**), 0.01 - 0.05 (*), and > 0.05 (n.s.). Ovary sections of wild-type (pre- and post-CG (cg) accumulation at the cortex, just below the vitelline envelope (ve). F,G) and mutant (pre- and post-CG accumulation). H,I) Ovaries showing that CGs (arrowheads) are aligned at the cortex, and yolk (y) morphology is normal in aura oocytes.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development